Poster Abstract
12. ISOLATION AND CHARACTERIZATION OF LACTOBACILLUS SPECIES FROM INTESTINAL CONTENTS OF CASPIAN SEA STURGEON
Mahdi Ghanbari and Mansoureh Jami
Institute of Hamun International Wetland, Zabol University, Zabol, Iran. Tel: +98542-2236961-2, Fax: +98542-2226765
ghanbari.msc@gmail.com
Lactic acid bacteria are gram-positive, non-sporulating and catalase negative rods or cocci that ferment various carbohydrates mainly to lactate and acetate. Most of the evidences come from salmonid species There are no reports on the presence of Lactobacillus in the intestines of sturgeon fish inhabiting Caspian sea The aim of the present work was to make a survey on the presence of lactobacilli in the intestinal content of beluga (Huso huso) and Persian sturgeon (Acipenser persicus), two high marketing values species of Caspian sea sturgeon fish.
After dissecting the fish, 1 g of the intestinal tract content of each fish was removed under aseptic condition and placed into previously weighed flasks containing storage medium.. Afterwards serial dilutions were spread on plates of selective media and incubated at the approperiate conditions. Eighty four strains were randomly selected for identification procedures based on the phenotypical characteristics. Counts of intestinal lactobacilli for Persian sturgeon and beluga were detected at the range of approximately 105.3 to 106.4 cfu/g, respectively ( Table 1, Table 2). The physiological and biochemical characterization of Lactobacillus isolates and the presumptive Lactobacillus species found in two fish species are shown in Table 2. From 84 isolates, 2 metabolic groups of Lactobacillus were recovered: facultative and obligate heterofermentatives. L. sakei and L. plantarum were the most often found isolates.
Knowledge on the presence of Lactobacillus as a natural flora in fish may lead to further applications to improve fish health. Consequensly, the discovered lactobacilli in this study can be candidates as probiotic bacteria. They should resist processing and storage conditions and be alive and active even after gastrointestinal passage.
Table 1: Average bacterial counts of intestinal bacteria (Log cfu/g of intestinal content) for Persian sturgeon and beluga in different media
Fish species |
No. |
CAB
(cfu/g) |
LAMVAB
(cfu/g) |
MRS 4.2
(cfu/g) |
Acipenser persicus |
12 |
7.84 |
5.32 |
4.85 |
Huso huso |
10 |
8.21 |
6.45 |
5.64 |
CAB: Columbia blood agar; LAMVAB: Lactobacillus spp. Anaerobic MRS with Vancomycin and Bromocresol green; MRS 4.2: deMan, Rogosa and Sharp
Table 2: Lactobacillus species isolated from the intestines of sturgeon fish
Presumptive
Lactobacillus species |
L. sakei |
L. plantarum |
L. coryneformis |
L. alimentarius |
L. brevis |
L. casei |
L. oris |
Acipenser persicus |
** |
** |
* |
** |
- |
** |
* |
Huso huso |
** |
* |
- |
* |
** |
* |
* |
* = Presence of lactobacilli. ** = High number of lactobacilli presence
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