Poster Abstract
30. TRANSCRIPTOMIC PROFILES OF JAPANESE MEDAKA (ORYZIAS LATIPES) IN RESPONSE TO ALKALINITY STRESS
Zong Li Yao*, Qi Fang Lai , Kai Zhou, Hui Wang
East China Sea Fisheries Research Institute, 300 Jungong Road, Shanghai, 200090, P. R. China
zlyao@126.com
Alkalinity stress is considered to be one of the major stressors for fish in saline-alkali water. Thus, it is of great significance from both aquaculture and physiological viewpoint to understand the molecular genetic response of aquatic organisms to alkalinity stress. The objective of this study is to determine genome-wide gene expression profiles to better understand the physiology response of medaka (Oryzias latipes) to high carbonate alkalinity stress. In lab-based cultures, adult fish were exposed to freshwater and high carbonate alkalinity water .We design a microarray containing 26429 genes and describe our experimental results for measuring gene expression changes in the gill of carbonate alkalinity stress exposed fish. Using T-test methods, we determined that 512 genes were up regulated and 501 genes were down regulated in the gill of medaka exposed to carbonate alkalinity stress. These differentially expressed genes can be divided into a number of biological gene ontology groups related to multicellular organismal process, response to stimulus, developmental process, metabolic process, catalytic activity, electron carrier activity, immune system process, extracellular region and transcription regulator activity. Biological pathways, mTOR signalling pathway, nitrogen metabolism, MAPK signalling pathway, calcium signalling pathway, GnRH signalling pathway etc. were significantly regulated. Alkalinity stress stimulates the energy and ion regulated pathway, at the same time slows down the pathways related to immune system and reproduction of medaka.
Figure 1. Number of gene ontology groups of medaka differentially expressed genes exposed to alkalinity stress (MF is the abbreviation for molecular function, BP for biological process and CC for cellular component)
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